Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles
Identifieur interne : 000622 ( Main/Exploration ); précédent : 000621; suivant : 000623Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles
Auteurs : Hanbi Kim ; Minseon Park [Corée du Sud] ; Joonki Hwang ; Jin Hwa Kim ; Doo-Ryeon Chung [Corée du Sud] ; Kyu-Sung Lee [Corée du Sud] ; Minhee Kang [Corée du Sud]Source :
- ACS Sensors [ 2379-3694 ] ; 2019.
Descripteurs français
- KwdFr :
- Cadres ouverts de lecture (génétique), Colorimétrie (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Disulfures (), Limite de détection, Nanoparticules métalliques (), Or (), Protéines de l'enveloppe virale (génétique), Techniques de biocapteur ().
- MESH :
- génétique : Cadres ouverts de lecture, Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines de l'enveloppe virale.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- Colorimétrie, Disulfures, Limite de détection, Nanoparticules métalliques, Or, Techniques de biocapteur.
English descriptors
- KwdEn :
- Biosensing Techniques (methods), Colorimetry (methods), Disulfides (chemistry), Gold (chemistry), Limit of Detection, Metal Nanoparticles (chemistry), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Open Reading Frames (genetics), Viral Envelope Proteins (genetics).
- MESH :
- chemical , chemistry : Disulfides, Gold.
- chemistry : Metal Nanoparticles.
- genetics : Middle East Respiratory Syndrome Coronavirus, Open Reading Frames, Viral Envelope Proteins.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Biosensing Techniques, Colorimetry.
- Limit of Detection.
Abstract
Worldwide outbreaks of infectious diseases necessitate the development of rapid and accurate diagnostic methods. Colorimetric assays are a representative tool to simply identify the target molecules in specimens through color changes of an indicator (e.g., nanosized metallic particle, and dye molecules). The detection method is used to confirm the presence of biomarkers visually and measure absorbance of the colored compounds at a specific wavelength. In this study, we propose a colorimetric assay based on an extended form of double-stranded DNA (dsDNA) self-assembly shielded gold nanoparticles (AuNPs) under positive electrolyte (e.g., 0.1 M MgCl2) for detection of Middle East respiratory syndrome coronavirus (MERS-CoV). This platform is able to verify the existence of viral molecules through a localized surface plasmon resonance (LSPR) shift and color changes of AuNPs in the UV–vis wavelength range. We designed a pair of thiol-modified probes at either the 5′ end or 3′ end to organize complementary base pairs with upstream of the E protein gene (upE) and open reading frames (ORF) 1a on MERS-CoV. The dsDNA of the target and probes forms a disulfide-induced long self-assembled complex, which protects AuNPs from salt-induced aggregation and transition of optical properties. This colorimetric assay could discriminate down to 1 pmol/μL of 30 bp MERS-CoV and further be adapted for convenient on-site detection of other infectious diseases, especially in resource-limited settings.
Url:
DOI: 10.1021/acssensors.9b00175
PubMed: 31062580
PubMed Central: 7119221
Affiliations:
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Le document en format XML
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Using Gold Nanoparticles</title><author><name sortKey="Kim, Hanbi" sort="Kim, Hanbi" uniqKey="Kim H" first="Hanbi" last="Kim">Hanbi Kim</name><affiliation><nlm:aff wicri:cut=" and" id="aff1a">Smart Healthcare & Device Research Center</nlm:aff></affiliation></author><author><name sortKey="Park, Minseon" sort="Park, Minseon" uniqKey="Park M" first="Minseon" last="Park">Minseon Park</name><affiliation><nlm:aff wicri:cut=" and" id="aff1a">Smart Healthcare & Device Research Center</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology),<institution>Sungkyunkwan University</institution>, Seoul,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hwang, Joonki" sort="Hwang, Joonki" uniqKey="Hwang J" first="Joonki" last="Hwang">Joonki Hwang</name><affiliation><nlm:aff wicri:cut=" and" id="aff1a">Smart Healthcare & Device Research Center</nlm:aff></affiliation></author><author><name sortKey="Kim, Jin Hwa" sort="Kim, Jin Hwa" uniqKey="Kim J" first="Jin Hwa" last="Kim">Jin Hwa Kim</name><affiliation><nlm:aff wicri:cut=" and" id="aff1a">Smart Healthcare & Device Research Center</nlm:aff></affiliation></author><author><name sortKey="Chung, Doo Ryeon" sort="Chung, Doo Ryeon" uniqKey="Chung D" first="Doo-Ryeon" last="Chung">Doo-Ryeon Chung</name><affiliation wicri:level="1"><nlm:aff id="aff1a">Center for Infection Prevention and Control,<institution>Samsung Medical Center</institution>, Seoul,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Asia Pacific Foundation for Infectious Diseases (APFID)</institution>, Seoul,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation><nlm:aff wicri:cut=" and" id="aff5a">Division of Infectious Diseases, Department of Internal Medicine</nlm:aff><wicri:noCountry code="subfield">Department of Internal Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Lee, Kyu Sung" sort="Lee, Kyu Sung" uniqKey="Lee K" first="Kyu-Sung" last="Lee">Kyu-Sung Lee</name><affiliation><nlm:aff wicri:cut=" and" id="aff1a">Smart Healthcare & Device Research Center</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology),<institution>Sungkyunkwan University</institution>, Seoul,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5a">Department of Urology,<institution>Samsung Medical Center, Sungkyunkwan University School of Medicine</institution>, Seoul,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kang, Minhee" sort="Kang, Minhee" uniqKey="Kang M" first="Minhee" last="Kang">Minhee Kang</name><affiliation><nlm:aff wicri:cut=" and" id="aff1a">Smart Healthcare & Device Research Center</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology),<institution>Sungkyunkwan University</institution>, Seoul,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">ACS Sensors</title><idno type="eISSN">2379-3694</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>Colorimetry (methods)</term><term>Disulfides (chemistry)</term><term>Gold (chemistry)</term><term>Limit of Detection</term><term>Metal Nanoparticles (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Open Reading Frames (genetics)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cadres ouverts de lecture (génétique)</term><term>Colorimétrie ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Disulfures ()</term><term>Limite de détection</term><term>Nanoparticules métalliques ()</term><term>Or ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Techniques de biocapteur ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Disulfides</term><term>Gold</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Open Reading Frames</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Colorimetry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Limit of Detection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Colorimétrie</term><term>Disulfures</term><term>Limite de détection</term><term>Nanoparticules métalliques</term><term>Or</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p content-type="toc-graphic"><graphic xlink:href="se9b00175_0005" id="ab-tgr1"></graphic></p><p>Worldwide
outbreaks of infectious diseases necessitate the development
of rapid and accurate diagnostic methods. Colorimetric assays are
a representative tool to simply identify the target molecules in specimens
through color changes of an indicator (e.g., nanosized metallic particle,
and dye molecules). The detection method is used to confirm the presence
of biomarkers visually and measure absorbance of the colored compounds
at a specific wavelength. In this study, we propose a colorimetric
assay based on an extended form of double-stranded DNA (dsDNA) self-assembly
shielded gold nanoparticles (AuNPs) under positive electrolyte (e.g.,
0.1 M MgCl<sub>2</sub>) for detection of Middle East respiratory syndrome
coronavirus (MERS-CoV). This platform is able to verify the existence
of viral molecules through a localized surface plasmon resonance (LSPR)
shift and color changes of AuNPs in the UV–vis wavelength range.
We designed a pair of thiol-modified probes at either the 5′
end or 3′ end to organize complementary base pairs with upstream
of the E protein gene (upE) and open reading frames (ORF) 1a on MERS-CoV.
The dsDNA of the target and probes forms a disulfide-induced long
self-assembled complex, which protects AuNPs from salt-induced aggregation
and transition of optical properties. This colorimetric assay could
discriminate down to 1 pmol/μL of 30 bp MERS-CoV and further
be adapted for convenient on-site detection of other infectious diseases,
especially in resource-limited settings.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Morens, D M" uniqKey="Morens D">D. M. Morens</name></author><author><name sortKey="Fauci, A S" uniqKey="Fauci A">A. S. Fauci</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tallury, P" uniqKey="Tallury P">P. Tallury</name></author><author><name sortKey="Malhotra, A" uniqKey="Malhotra A">A. Malhotra</name></author><author><name sortKey="Byrne, L M" uniqKey="Byrne L">L. M. Byrne</name></author><author><name sortKey="Santra, S" uniqKey="Santra S">S. Santra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, J W" uniqKey="Kim J">J. W. Kim</name></author><author><name sortKey="Kim, J H" uniqKey="Kim J">J. H. Kim</name></author><author><name sortKey="Chung, S J" uniqKey="Chung S">S. J. 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Leyten</name></author><author><name sortKey="Groeneveld, P" uniqKey="Groeneveld P">P. Groeneveld</name></author><author><name sortKey="Jansen, C" uniqKey="Jansen C">C. Jansen</name></author><author><name sortKey="Jonges, M" uniqKey="Jonges M">M. Jonges</name></author><author><name sortKey="Raj, S" uniqKey="Raj S">S. Raj</name></author><author><name sortKey="Thurkow, I J E" uniqKey="Thurkow I">I. J. E. Thurkow</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Hwang, Joonki" sort="Hwang, Joonki" uniqKey="Hwang J" first="Joonki" last="Hwang">Joonki Hwang</name><name sortKey="Kim, Hanbi" sort="Kim, Hanbi" uniqKey="Kim H" first="Hanbi" last="Kim">Hanbi Kim</name><name sortKey="Kim, Jin Hwa" sort="Kim, Jin Hwa" uniqKey="Kim J" first="Jin Hwa" last="Kim">Jin Hwa Kim</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Park, Minseon" sort="Park, Minseon" uniqKey="Park M" first="Minseon" last="Park">Minseon Park</name></noRegion><name sortKey="Chung, Doo Ryeon" sort="Chung, Doo Ryeon" uniqKey="Chung D" first="Doo-Ryeon" last="Chung">Doo-Ryeon Chung</name><name sortKey="Chung, Doo Ryeon" sort="Chung, Doo Ryeon" uniqKey="Chung D" first="Doo-Ryeon" last="Chung">Doo-Ryeon Chung</name><name sortKey="Kang, Minhee" sort="Kang, Minhee" uniqKey="Kang M" first="Minhee" last="Kang">Minhee Kang</name><name sortKey="Lee, Kyu Sung" sort="Lee, Kyu Sung" uniqKey="Lee K" first="Kyu-Sung" last="Lee">Kyu-Sung Lee</name><name sortKey="Lee, Kyu Sung" sort="Lee, Kyu Sung" uniqKey="Lee K" first="Kyu-Sung" last="Lee">Kyu-Sung Lee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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